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rabbit monoclonal antibodies against zo 1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal antibodies against zo 1
    Rabbit Monoclonal Antibodies Against Zo 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal antibodies against zo 1/product/Cell Signaling Technology Inc
    Average 96 stars, based on 283 article reviews
    rabbit monoclonal antibodies against zo 1 - by Bioz Stars, 2026-02
    96/100 stars

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    Vitamin D 3 re-establishes iBRB integrity through modulation of VE-cadherin and <t>ZO-1.</t> HRECs were pretreated with vitamin D 3 (1 μM) for 24 h and subsequently co-treated with HG (40 mM) for other 48 h. Vitamin D 3 increased the expression of VE-cadherin and ZO-1 proteins, which were significantly reduced by HG. Representative images for VE-cadherin (A,B,C) and ZO-1 (E,F,G) expression in HRECs after treatment with HG and vitamin D 3 . VE-cadherin and ZO-1 were labeled with FITC (green); nuclei were labeled with DAPI (blue). Images were acquired at × 40 magnification. Scale bar: 10 µm. Fluorescence semi-quantification of VE-cadherin (D) and ZO-1 (H) protein (mean grey levels). Values are reported as mean ± SD; n = 4. Data were analyzed by one-way ANOVA and Tukey post-hoc test for multiple comparisons. * p < 0.05 vs. control; † p < 0.05 vs. HG.
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    Vitamin D 3 re-establishes iBRB integrity through modulation of VE-cadherin and ZO-1. HRECs were pretreated with vitamin D 3 (1 μM) for 24 h and subsequently co-treated with HG (40 mM) for other 48 h. Vitamin D 3 increased the expression of VE-cadherin and ZO-1 proteins, which were significantly reduced by HG. Representative images for VE-cadherin (A,B,C) and ZO-1 (E,F,G) expression in HRECs after treatment with HG and vitamin D 3 . VE-cadherin and ZO-1 were labeled with FITC (green); nuclei were labeled with DAPI (blue). Images were acquired at × 40 magnification. Scale bar: 10 µm. Fluorescence semi-quantification of VE-cadherin (D) and ZO-1 (H) protein (mean grey levels). Values are reported as mean ± SD; n = 4. Data were analyzed by one-way ANOVA and Tukey post-hoc test for multiple comparisons. * p < 0.05 vs. control; † p < 0.05 vs. HG.

    Journal: Frontiers in Pharmacology

    Article Title: Vitamin D 3 preserves blood retinal barrier integrity in an in vitro model of diabetic retinopathy

    doi: 10.3389/fphar.2022.971164

    Figure Lengend Snippet: Vitamin D 3 re-establishes iBRB integrity through modulation of VE-cadherin and ZO-1. HRECs were pretreated with vitamin D 3 (1 μM) for 24 h and subsequently co-treated with HG (40 mM) for other 48 h. Vitamin D 3 increased the expression of VE-cadherin and ZO-1 proteins, which were significantly reduced by HG. Representative images for VE-cadherin (A,B,C) and ZO-1 (E,F,G) expression in HRECs after treatment with HG and vitamin D 3 . VE-cadherin and ZO-1 were labeled with FITC (green); nuclei were labeled with DAPI (blue). Images were acquired at × 40 magnification. Scale bar: 10 µm. Fluorescence semi-quantification of VE-cadherin (D) and ZO-1 (H) protein (mean grey levels). Values are reported as mean ± SD; n = 4. Data were analyzed by one-way ANOVA and Tukey post-hoc test for multiple comparisons. * p < 0.05 vs. control; † p < 0.05 vs. HG.

    Article Snippet: Cells were then incubated overnight at 4°C with primary antibody against ZO-1 (dilution 1:100, rabbit monoclonal; catalog n. 61-7300, Life Technology, Monza, Italy).

    Techniques: Expressing, Labeling, Fluorescence, Control

    Vitamin D 3 induces VE-cadherin and ZO-1 mRNA expression. VE-cadherin (A) and ZO-1 (B) mRNA levels after treatment with vitamin D 3 and HG for 48 h. Values are reported as mean ± SD; n = 4. Data were analyzed by one-way ANOVA and Tukey post-hoc test for multiple comparisons. * p < 0.05 vs. control; † p < 0.05 vs. HG.

    Journal: Frontiers in Pharmacology

    Article Title: Vitamin D 3 preserves blood retinal barrier integrity in an in vitro model of diabetic retinopathy

    doi: 10.3389/fphar.2022.971164

    Figure Lengend Snippet: Vitamin D 3 induces VE-cadherin and ZO-1 mRNA expression. VE-cadherin (A) and ZO-1 (B) mRNA levels after treatment with vitamin D 3 and HG for 48 h. Values are reported as mean ± SD; n = 4. Data were analyzed by one-way ANOVA and Tukey post-hoc test for multiple comparisons. * p < 0.05 vs. control; † p < 0.05 vs. HG.

    Article Snippet: Cells were then incubated overnight at 4°C with primary antibody against ZO-1 (dilution 1:100, rabbit monoclonal; catalog n. 61-7300, Life Technology, Monza, Italy).

    Techniques: Expressing, Control